Project director: Barrie Robison

To define the recombinational landscape of the 12 chromosomes, we need to finalize the genetic map of D. pulex using our arsenal of microsatellite markers. This project is optimally staged for completion. First, a small F2 mapping panel comprised of selfed progenies from crosses between genetically divergent Oregon populations is currently being genotyped, while their numbers are increased by hatching more offspring. Second, most of the requisite markers have been isolated and their flanking regions sequenced. Our goal, however, is to assign 1,000 markers to the genetic map and approximately 30% of the loci tested are uninformative in this specific mapping panel. Therefore, we are supplementing the Oregon panel with another mapping panel under construction using parents from mid-western US populations. Markers and mapping panels are now also being developed for D. magna, whose genome contains only 10 chromosomes.

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