Project director: Kelley Thomas

A combined physical and genetic map is essential to estimate the recombination rates per physical distance in various parts of the genome and to assemble overlapping genome sequence data into entire chromosomal arms. To this end, BAC clones are digested using restriction enzymes and their fingerprints are then compared to recognize overlapping clones, leading to a tiling path of BACs. After robotically spotting the BAC library onto nylon filters, gaps between each group will be bridged by using sequence specific probes (including probes designed from unique regions flanking genetically mapped microsatellite markers) to identify all clones with which they would hybridize. The resultant product will be the organization of the BAC contigs into chromosome-scaffolds from which regional recombination rates will be established. Because ~30% of the microsatellite markers are contained within some exonic DNA, a few hundred genes will be directly localized with no sequencing. We also intend to probe the tiled BAC library with gene specific probes designed from the EST sequencing project, thus maximizing the information content of our genomic maps.

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