wFleaBase: The Daphnia Genomics Information Systems

wFleaBase is a database with the necessary infrastructure to curate, archive and share genetic, molecular and functional genomics data and protocols via the world-wide-web. It includes data from all species of the genus Daphnia, yet the primary species are D. pulex and D. magna. Although now in its adolescence, wFleaBase currently contains search functions and information to extract sequences from microsatellite DNA, cDNA and random genomic surveys. We have deposited the first set of 538 characterized microsatellite primer sequences and their optimal amplification conditions. More than 1500 such markers should be available at the close of the mapping projects. This database will also serve as a repository of raw and transformed microarray images, with sufficient experimental details following Microarray Gene Expression Data (MGED) Society standards, as these experiments produce a deluge of potentially valuable data beyond the specific focus of individual studies. wFleaBase aims to provide genomic information that can serve a large ecological and evolutionary research community. Our challenge is to rapidly expand its content and to ultimately integrate genetic and functional genomic information with population-level responses to environmental challenges.

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